KMID : 0861020170320040025
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Korea Journal of Herbology 2017 Volume.32 No. 4 p.25 ~ p.32
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A Study on Change of Marker Compounds and Biological Activity in Chungsimyeonja-eum Decoction Depending on A Storage Temperature and Periods
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Yoo Sae-Room
Ha Hye-Kyung Lee Na-Ri Shin Hyeun-Kyoo Seo Chang-Seob
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Abstract
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Objectives : Chungsimyeonja-eum (CSYJE; Qingxinlianzi-yin in Chinese; Seishinrenshi-in in Japanese), a traditional herbal medicine, has been used for treating mouth dryness, hyperuresis. This study was designed to determine preservation period of CSYJE. We investigated the stability and biological activity of CSYJE depending on the preservation temperature and periods.
Methods : CSYJE decoction was preserved for 0-6 or 12 months at room temperature (RT, 23¡¾1¡É) or refrigeration (4¡É). To evaluate the stability of CSYJE decoction, pH and dissolved solids content were measured. High-performance liquid chromatography (HPLC) analysis was performed to determine marker compounds-liquiritin apioside, liquiritin, baicalin, wogonoside, and glycyrrhizin-in CSYJE. To determine anti-inflammatory effect of CSYJE, prostaglandin E2 (PGE2) was measured in LPS-stimulated RAW 264.7 macrophages.
Results : Both at RT and 4¡É, pH was decreased depending on the preservation periods. There was no changes in dissolved solids content depending on the preservation temperature and periods. Both at RT and 4¡É, the contents of liquiritin apioside and liquiritin were slightly increased at 1 month of storage. The level of baicalin was decreased time-dependently and the disappearance rate at RT is larger than at 4¡É. CSYJE inhibited PGE2 production in LPS-stimulated RAW 264.7 cells and maintained inhibitory effect by 12 months both at RT and 4¡É.
Conclusions : Based on the disappearance rate of the baicalin in CSYJE, the preservation period is recommended within 8 months for RT and 12 months for 4¡É.
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KEYWORD
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Chungsimyeonja-eum, HPLC, anti-inflammation, storage, temperature
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